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Cyathostomins are the most common and important group of large intestine nematodes, infecting horses worldwide. The current control strategy is associated with the development of anthelmintic resistance, which has been reported worldwide. Therefore, experiments with this family of parasites have become progressively important to provide their monitoring and control strategies.
The aim of the present study was to propose a faster and more economic assay for isolation of genomic DNA from the adult stage of Cyathostomin parasites than reported. Genomic DNA was isolated from ten individual female adult parasites using a standardized procedure developed.
Then, extraction from ten individual female was carried out by another DNA extraction method. DNA concentration from both methods were measured and compared. We obtained a good DNA quality with this standardized procedure.
As a result of this analysis, we propose a modified phenol-chloroform method, which will contribute to assays that require DNA extraction from adult worms for genomic DNA sequences of cyathostomin, or species-specific identification.
Home Comparison of Two Methods for Short Communication. Curitiba, PR, Brazil 2. Laboratory of Biochemistry, University of Camaguey. Camaguey, Cuba 3. Belo Horizonte, MG. Acta Vet Eurasia ; Keywords : DNA extraction , genomic material , horse , Strongylida. Read: 21 Downloads: 40 Published: 22 December Volume 43, Issue 2, June Previous Article. Advanced Search Search. Email Submit.